[Czech Republic] Secondary metabolites are organic compounds that are not directly involved in the normal growth or reproduction of organisms. However secondary metabolites are indispensable in plants exposed to abiotic and biotic stress and therefore they are indirectly essential for growth in natural, mostly extreme, conditions. Small RNAs are other key players in plant response to stress conditions. Both the extreme changes of the weather and toxic waste are currently the major problem in the crop plant growing. Moreover, recently with development of nanotechnology and nanofabrication, the phytotoxicity of nanoparticles has been also intensively studied. On the other hand, nanoparticles play an important role in phytonanomedicine.
In the near future, increasingly difficult conditions for sustainable crop production can be expected. Hence, understanding the molecular basis of interplay between small RNAs and biosynthesis of secondary metabolites and identification of key factors involved in their pathways are fundamental knowledge to develop of resistant crop plants.
Because the biosynthesis of secondary metabolites is restricted to selected plant groups, the project will be focused to different plants species including Hordeum, Zea, Nicotiana and Selengia genus, and also algae and lichen. Otherwise Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system will be used for the investigation of the regulation of secondary metabolites biosynthesis.
The principal objectives of the project are as follows: i) Analysis of metabolome in mentioned plants, algae and lichen in different conditions such as drought, nutrient starvation, heavy metals and nanoparticles. ii) Analysis of transcriptome and micro RNA sequencing in tolerant species. iii) Design of probes from the sequencing data and analysis of specific micro-RNAs and gene expression using both the classical approaches and the electrochemical microarray. iiii) On the basis of the obtained data, applying the CRISPR/Cas9 method to improve plant defense against stress.
H2020 call: SFS-10-2017: Research and approaches for emerging diseases in plants and terrestrial livestock
Call deadlines (two-stage): 14/2/17 and 13/09/17
Deadline for EoIs: 31/10/16
Partner expertise sought:
- Type of partner sought: The university is looking for partners (research institutions and businesses) with interest in the topic, including laboratory groups primary focused on small RNAs biology, bioinformatics and with experience in CRISPR/Cas9 method in plants.
- Experienced project leader is also sought.
- Specific area of activity of the partner: Universities, Research Organization and private businesses are sought to join this project. Potential partners should have experience with small RNAs biology, bioinformatics and in CRISPR/Cas9 method in plants. Experienced project leader is also sought.
Advantages & innovations:
The proposing laboratory has extensive experience with modern analytical facilities and methods for determination of secondary metabolites, molecular biology, cultivation of plant cell culture and microalgae, scanning electron microscopes and transmission electron microscopes, electrochemical microarray and capillary chip electrophoresis.
It is also experienced as an H2020 project partner.
Programme – Call:
Evaluation scheme: Two-stage call with following deadlines – first stage February 14th, 2017 – second stage September 13th, 2017
Acronym: Plant secondary metabolites and small RNAs: the tools in plant defense system
Coordinator required: Yes